Publications

General Acoustofluidics

Acoustophoresis in polymer-based microfluidic devices: Modeling and experimental validation

Lickert F, Ohlin M, Bruus H and Ohlsson, P.
The Journal of the Acoustical Society of America, 2021,149, 4281

Acoustofluidic hematocrit determination

Petersson K., Jakobsson O., Ohlsson P., Augustsson P., Scheding S., Malm J., Laurell T.
Anal Chim Acta, 2018, 1000, 199-204, DOI: 10.1016/j.aca.2017.11.037

Continuous flow microfluidic separation and processing of rare cells and bioparticles found in blood – A review

Maria Antfolk, Thomas Laurell
Analytica Chimica Acta, 2017, 965, 9-35

Thousand-fold volumetric concentration of live cells with a recirculating acoustofluidic device

Jakobsson O., Oh S.S., Antfolk M., Eisenstein M., Laurell T. and H.T. Soh
Analytical Chemistry, 2015, 87, 8497-8502

Acoustic actuated fluorescence activated sorting of microparticles

Jakobsson O.J.E., Grenvall C., Nordin M., Evander M. and T. Laurell
Lab Chip, 2014, 14 (11), 1943-1950

Acoustofluidics 8: Applications of acoustophoresis in continuous flow microsystems

Lenshof A., Magnusson C. and T. Laurell
Lab on a chip, 2012, 12, 1210-1233

Acoustofluidics 20: Applications in acoustic trapping

Evander M. and J. Nilsson
Lab on a chip, 2012, 12, 4667-4676

Label-free somatic cell cytometry in raw milk using acoustophoresis

Grenvall C., Folkenberg J.R., Augustsson P. and T. Laurell
Cytometry Part A, 2012, 81A, 1076-1083

Emerging clinical applications of microchip based acoustophoresis

Lenshof A. and T. Laurell
Journal of Laboratory Automation, 2011, 16, 443-449

Review of cell and particle trapping in microfluidic systems

Nilsson J., Evander M., Hammarström B. and Laurell T.
Analytica Chimica Acta, 2009, 649, 141-157

Trapping of microparticles in the nearfield of an ultrasonic transducer

Lilliehorn T., Simu U., Nilsson M., Almqvist M., Stepinski T., Laurell T., Nilsson J., Johansson S.
Ultrasonics 2005, 43(5): 289-299

Acoustic Control Of Suspended Particles In Micro Fluidic Chips

Nilsson A., Petersson F., Jönsson H. and Laurell T.
Lab Chip, 2004, 4, 131-135

Acoustic Separation

Blood and stem cell separation

Acoustophoresis enables the label‐free separation of functionally different subsets of cultured bone marrow stromal cells

Franziska Olm, Hooi Ching Lim, Katharina Schallmoser, Dirk Strunk, Thomas Laurell and Stefan Scheding
Cyto, 2020, Accepted Manuscript

Label-free separation of leukocyte subpopulations using high throughput multiplex acoustophoresis

Anke Urbansky, Franziska Olm, Stefan Scheding, Thomas Laurell and Andreas Lenshof
Lab Chip, 2019, 19, 1406

Rapid and effective enrichment of mononuclear cells from blood using acoustophoresis

A. Urbansky, P. Ohlsson, A. Lenshof, F. Garofalo, S.Scheding and T. Laurell
Scientific Reports, 2017, 7:17161

Acoustophoretic purification of platelets: feasibility and impact on platelet activation and function

Bohec, P. et al.
Platelets 30, 174–180 (2019)

Efficient purification of CD4+ lymphocytes from peripheral blood progenitor cell products using affinity bead acoustophoresis

Lenshof A., Jamal A., Dykes J.,Urbansky A., Åstrand-Grundström I., Laurell T. and S. Scheding
Cytometry Part A, 2014, 85A, 933-941

Affinity Bead Mediated Enrichment of CD8+ Lymphocytes from Peripheral Blood Progenitor Cell Products using Acoustophoresis

Urbansky A., Lenshof A., Dykes, J., Laurell T., and Scheding S.
Micromachines, 2016, 7, 101

Concurrent isolation of lymphocytes and granulocytes using prefocused free flow acoustophoresis

Grenvall C., Magnusson C., Lilja H. and T. Laurell
Analytical Chemistry, 2015, 87, 5596-5604

Integrated acoustic immunoaffinity-capture (IAI) platform for detection of PSA from whole blood samples

Tajudin, A. A. et al.
Lab Chip 13, 1790–1796 (2013)

Efficient removal of platelets from peripheral blood progenitor cell products using a novel micro-chip based acoustophoretic platform

Dykes, J. et al.
PLoS One 6, e23074 (2011)

Circulating tumor cells

Clinical scale cell-surface marker independent, acoustic microfluidic enrichment of cancer cells from blood

Cecilia Magnusson, Per Augustsson, Andreas Lenshof, Yvonne Ceder, Thomas Laurell and Hans Lilja
Anal Chem, 2017, 89, 22, 11954-11961

Label-free neuroblastoma cell separation from hematopoietic progenitor cell products using acoustophoresis – towards cell processing of complex biological samples

Franziska Olm, Anke Urbansky, Josefina H. Dykes, Thomas Laurell, and Stefan Scheding
Scientific Report, 2019,9, 8777 (2019), doi:10.1038/s41598-019-45182-3

Reducing WBC background in cancer cell separation products by negative acoustic contrast particle immuno-acoustophoreis

Cushing K., Undvall E., Ceder Y., Lilja H., and T. Laurell
Anal Chim Acta, 2018, 1000, 256-264, DOI: 10.1016/j.aca.2017.11.064

Label-free single-cell separation and imaging of cancer cells using an integrated microfluidic system

Maria Antfolk, Soo Hyeon Kim, Saori Koizumi, Teruo Fujii, Thomas Laurell
Scientific Reports, 2017, 7, 46507

Acoustofluidic, Label-free Separation and Simultaneous Concentration of Rare Tumor Cells from White Blood Cells

Antfolk M., Magnusson C., Augustsson P., Lilja H. and T. Laurell
Analytical Chemistry, 2015, 87, 9322-9328

A single inlet two-stage scoustophoresis enabling tumor cell enrichment from white blood cells

Antfolk M., Antfolk C., Lilja H., Laurell T. and P. Augustsson
Lab on a chip, 2015, 15, 2102-2109

Microfluidic, Label-free enrichment of prostate cancer cells in blood based on acoustophoresis

Augustsson P., Magnusson C., Nordin M., Lilja H. and T. Laurell
Analytical Chemistry, 2012, 84, 7954-7962

Cell wash

Acoustophoretic removal of proteins from blood components

Tenje M., Lundgren M.N., Swärd-Nilsson A-M., Kjeldsen-Kragh J., Åberg L. and A. Lenshof
Biomedical Microdevices, 2015, 17:95

Particle separation using ultrasound can radically reduce embolic load to brain after cardiac surgery

Jönsson, H. et al.
Ann. Thorac. Surg. 78, 1572–1577 (2004)

Viability

Label-free concentration of viable neurons, hESC and cancer cells by means of acoustophoresis

Marina C. Zalis, Juan F. Reyes, Per Augustsson, Staffan Holmqvist, Laurent Roybon, Thomas Laurell, Tomas Deierborg
Integrative Biology, 2016, 8, 332-340

Microchannel acoustophoresis does not impact survival or function of microglia, leukocytes or tumor cells

Burguillos M.A., Magnusson C., Nordin M., Lenshof A., Augustsson P., Hansson M.J., Elmér E., Lilja H., Brundin P., Laurell T. and T. Deierborg
PLoS ONE, 2013, 8, e6423

Acoustophoretic sorting of viable mammalian cells in a microfluidic device

Yang, A. H. J. J. & Soh, H. T.
Anal. Chem. 84, 10756–10762 (2012)

Bacteria and algae

Integrated acoustic separation, enrichment and microchip PCR detection of bacteria from blood for rapid sepsis diagnostics

Pelle Ohlsson, Mikael Evander, Klara Petersson, Lisa Mellhammar, Ari Lehmusvuori, Ulla Karhunend, Minna Soikkeli, Titta Seppä, Emilia Tuunainen, Anni Spangar, Piia von Lode, Kaisu Rantakokko-Jalava, Gisela Otto, Stefan Scheding, Tero Soukka, Saara Wittfooth and Thomas Laurell
Anal Chem, 2016, 88, 19, 9403-9411

Gradient acoustic focusing of sub-micron particles for separation of bacteria from blood lysate

Van Assche, D., Reithuber, E., Qiu, W., Laurell, T., Henriques-Normark, B., Mellroth, P., Ohlsson, P., & Augustsson, P
Scientific Reports. 2020;10(1):1-13. doi:10.1038/s41598-020-60338-2

Acoustic impedance matched buffers enable separation of bacteria from blood cells at high cell concentrations

Ohlsson P., Petersson K., Augustsson P. and Laurell T.
Scientific Reports, 2018, 8:9156, DOI:10.1038/s41598-018-25551-0

Acoustofluidic harvesting of microalgae on a single chip

Park, J. W. et al.
Biomicrofluidics 10, 1–11 (2016)

Focusing of sub-micrometer particles and bacteria enabled by two-dimensional acoustophoresis

Antfolk M., Barkholt Muller P., Augustsson P, Bruus H. and T. Laurell
Lab Chip, 2014, 14(15), 2791-2799

Acoustic Trapping

Extracellular vesicles

Microvesicles in plasma reflect coronary flow reserve in patients with cardiovascular disease

Bryl-Górecka P, James K, Torngren K, Haraldsson I, Gan LM, Svedlund S, Olde B, Laurell T, Omerovic E, Erlinge D.
Am J Physiol Heart Circ Physiol. 2021

High-Throughput and Automated Acoustic Trapping of Extracellular Vesicles to Identify microRNAs With Diagnostic Potential for Prostate Cancer

Ku A, Fredsøe J, Sørensen KD, Borre M, Evander M, Laurell T, Lilja H, Ceder Y.
Front Oncol. 2021 Mar 25;11:631021

Development of acoustically isolated extracellular plasma vesicles for biomarker discovery in allogeneic hematopoietic stem cell transplantation

Lim, H.C., Soneji, S., Pálmason, R. et al.
Biomark Res 9, 6 (2021)

Bilberry Supplementation after Myocardial Infarction Decreases Microvesicles in Blood and Affects Endothelial Vesiculation

Paulina Bryl-Górecka, Ramasri Sathanoori, Lilith Arevström, Rikard Landberg, Cecilia Bergh, Mikael Evander, Björn Olde, Thomas Laurell, Ole Fröbert and David Erlinge.
Mol. Nutr. Food Res. 2020, 2000108.

Proteomic profiling of extracellular vesicles reveals additional diagnostic biomarkers for myocardial infarction compared to plasma alone

Olof Gidlöf, Mikael Evander, Melinda Rezeli, György Marko-Varga, Thomas Laurell, David Erlinge
Scientific Report, 2019, 9, 8991, DOI: doi.org/10.1038/s41598-019-45473-9

A urinary extracellular vesicle microRNA biomarker discovery pipeline; from automated urinary extracellular vesicle enrichment by acoustic trapping to microRNA sequencing

Anson Ku, Naveen Ravi, Minjun Yang, Mikael Evander, Thomas Laurell, Hans Lilja, Yvonne Ceder
PLOS ONE, 2019, doi: 10.1371/journal.pone.0217507

Effect of exercise on the plasma vesicular proteome: a methodological study compaing acoustic trapping and centrifugation

Bryl-Gorecka P., Sathanoori R., Al-Mashat M., Olde B., Jögi J., Evander M., Laurell T. and D. Erlinge
Lab Chip, 2018,18, 3101-3111, DOI: 10.1039/C8LC00686E

Acoustic enrichment of extracellular vesicles from biological fluids

Ku A., Lim HC, Evander M., Lilja H., Laurell T., Scheding S., and Y. Ceder
Anal Chem, 2018, 90, 8011-8019, DOI:10.1021/acs.analchem.8b00914

Comparative Proteomic Analysis of Extracellular Vesicles Isolated by Acoustic Trapping or Differential Centrifugation

Melinda Rezeli, Olof Gidlöf, Mikael Evander, Paulina Bryl-Górecka, Ramasri Sathanoori, Patrik Gilje, Krzysztof Pawłowski, Péter Horvatovich, David Erlinge, György Marko-Varga and Thomas Laurell
Anal. Chem. 2016, 88, 17, 8577-8586

Non-contact acoustic capture of microparticles from small plasma volumes

Evander M., Gidlof O., Olde B., Erlinge D. and T. Laurell
Lab on a chip, 2015, 15, 2588-2596

Cell trapping

Isolation of a Low Number of Sperm Cells from Female DNA in a Glass−PDMS−Glass Microchip via Bead Assisted Acoustic Differential Extraction

Kerui Xu, Charles P. Clark, Brian L. Poe, Jenny A. Lounsbury, Johan Nilsson, Thomas Laurell and James P. Landers
Analytical Chemistry 2019 5;91(3):2186-2191. doi: 10.1021/acs.analchem.8b04752

Non-contact acoustic cell trapping in disposable glass capillaries

Hammarström B., Evander M., Barbeau H., Bruzelius M., Larsson J., Laurell T. and J. Nilsson
Lab on a Chip, 2010, 10, 2251-2257

Acoustic Differential Extraction for Forensic Analysis of Sexual Assault Evidence

Norris J.V., Evander M., Horsman-Hall K.M., Nilsson J., Laurell T., Landers J.P., Acoustic
Analytical Chemistry, 2009, 81, 6089-6095

Non-invasive acoustic cell trapping in a microfluidic perfusion system for on-line bioassays

M. Evander, L. Johansson, T. Lilliehorn, J. Piskur, M. Lindvall , S. Johansson, M. Almqvist, T. Laurell and J. Nilsson
Analytical Chemistry, 2007, 79, 2984-2991

Bacteria

Integrated acoustic separation, enrichment and microchip PCR detection of bacteria from blood for rapid sepsis diagnostics

Pelle Ohlsson, Mikael Evander, Klara Petersson, Lisa Mellhammar, Ari Lehmusvuori, Ulla Karhunend, Minna Soikkeli, Titta Seppä, Emilia Tuunainen, Anni Spangar, Piia von Lode, Kaisu Rantakokko-Jalava, Gisela Otto, Stefan Scheding, Tero Soukka, Saara Wittfooth and Thomas Laurell
Anal Chem, 2016, 88, 19, 9403-9411

Acoustic trapping for bacteria identification in positive blood cultures with MALDI-TOF MS

Hammarstrom B., Nilson B., Laurell T., Nilsson J. and S. Ekstrom
Analytical Chemistry, 2014, 86, 10560-10567, DOI: 10.1021/ac502020f

Seed particle enabled acoustic trapping of bacteria and nanoparticles in continuous flow systems

Hammarström B., Laurell T. and J. Nilsson
Lab on a chip, 2012, 12, 4296-4304

Assay automation

Acoustic trapping as a generic non-contact incubation site for multiplex bead-based assays

Tenje, M. Hongyan X., Evander M. Hammarstrom B., Tojo A., Belak S., Laurell T. and N LeBlanc
Analytica Chimica Acta, 2015, 853, 682-688

Efficient sample preparation in immuno-matrix-assisted laser derorption/ionization mass spectrometry using acoustic trapping

Hammarström B., Yan H., Nilsson J. and S. Ekström
Biomicrofluidics, 2013, 7, 024107

AcouTrap

Microvesicles in plasma reflect coronary flow reserve in patients with cardiovascular disease

Bryl-Górecka P, James K, Torngren K, Haraldsson I, Gan LM, Svedlund S, Olde B, Laurell T, Omerovic E, Erlinge D.
Am J Physiol Heart Circ Physiol. 2021

High-Throughput and Automated Acoustic Trapping of Extracellular Vesicles to Identify microRNAs With Diagnostic Potential for Prostate Cancer

Ku A, Fredsøe J, Sørensen KD, Borre M, Evander M, Laurell T, Lilja H, Ceder Y.
Front Oncol. 2021 Mar 25;11:631021

Development of acoustically isolated extracellular plasma vesicles for biomarker discovery in allogeneic hematopoietic stem cell transplantation

Lim, H.C., Soneji, S., Pálmason, R. et al.
Biomark Res 9, 6 (2021)

Bilberry Supplementation after Myocardial Infarction Decreases Microvesicles in Blood and Affects Endothelial Vesiculation

Paulina Bryl-Górecka, Ramasri Sathanoori, Lilith Arevström, Rikard Landberg, Cecilia Bergh, Mikael Evander, Björn Olde, Thomas Laurell, Ole Fröbert and David Erlinge.
Mol. Nutr. Food Res. 2020, 2000108.

Proteomic profiling of extracellular vesicles reveals additional diagnostic biomarkers for myocardial infarction compared to plasma alone

Gidlöf et al.
Scientific Report, 2019, 9, 8991, DOI: doi.org/10.1038/s41598-019-45473-9

A urinary extracellular vesicle microRNA biomarker discovery pipeline; from automated urinary extracellular vesicle enrichment by acoustic trapping to microRNA sequencing

Ku et al.
PLOS ONE, 2019, doi: 10.1371/journal.pone.0217507

Effect of exercise on the plasma vesicular proteome: a methodological study compaing acoustic trapping and centrifugation

Bryl-Gorecka et al.
Lab Chip, 2018,18, 3101-3111, DOI: 10.1039/C8LC00686E

Acoustic enrichment of extracellular vesicles from biological fluids

Ku et al.
Anal Chem, 2018, 90, 8011-8019, DOI:10.1021/acs.analchem.8b00914

Comparative Proteomic Analysis of Extracellular Vesicles Isolated by Acoustic Trapping or Differential Centrifugation

Rezeli et al.
Anal. Chem. 2016, 88, 17, 8577-8586

Non-contact acoustic capture of microparticles from small plasma volumes

Evander et al.
Lab on a chip, 2015, 15, 2588-2596

AcouWash

AcouPlasma

Acoustofluidic hematocrit determination

Petersson et al.
Anal. Chim. Acta 1000, 199–204 (2018)
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