Technology

Moving cells with sound

The core technology of AcouSort is acoustofluidics. By using acoustofluidics, cells and particles can be separated and trapped in acoustic fields. These techniques can perform almost any operation that is normally done by centrifugation in the biology or clinical lab. With an automated flow we are able to handle small samples and low cell concentrations.

Acoustic separation

To separate cells, the sample is flushed through a microchannel, which typically is half a millimeter wide. An acoustic standing wave is generated across the channel that moves cells and particles, which are denser or less compressible than the surrounding fluid, to the pressure node in the center of the channel.

This way cells can be moved from one medium to another, concentrated, removed from a medium or different cell types can be separated if they differ in size, density or compressibility. All these operations can be performed in either the AcouWash standalone platform or in customized OEM solutions.  Applications may for example be to wash cells, separate plasma from blood for clinical diagnostics or to find a certain type of cells in a sample, such as bacteria in a blood sample.

acousort-1a

This way cells can be moved from one medium to another, concentrated, removed from a medium or different cell types can be separated if they differ in size, density or compressibility. All these operations can be performed in either the AcouWash standalone platform or in customized OEM solutions.  Applications may for example be to wash cells, separate plasma from blood for clinical diagnostics or to find a certain type of cells in a sample, such as bacteria in a blood sample.

Acoustic trapping

As shown in the animation video, cells and particles can also be captured and enriched from a medium using a local acoustic field. This also works for submicron particles, such as subcellular components and bacteria, if the acoustic trap is pre-loaded with larger seeding particles.

In the AcouTrap platform an acoustic trap is mounted as tip in a pipetting robot, so that cells and subcellular particles can be aspirated and enriched from and released into a 96-well-plate. This platform can be used to automate cell assays where cells are held in the acoustic field and exposed to reagents from the 96-well-plate. Also bacteria and extracellular vesicles can be trapped and enriched. The acoustic trap can also be customized for OEM solutions.

Do not hesitate to contact us to discuss if our products, technologies or OEM solutions can be used for your applications.