Chris Dunning is a user of AcouTrap at Lund University where he as an experimental scientist is doing research on how diseases such as Parkinson and Alzheimer spread. Traditional methods of isolating extracellular vesicles are time-consuming, and at Lund University he was introduced by clinician Oskar Hansson and Professor Thomas Laurell to the AcouTrap system, a new way of isolating the micro vesicles.
The AcouTrap is used by Chris Dunning on an almost daily basis. He is able to isolate particles of 50-100nm by using acoustophoresis.
In the beginning I was suspicious. Would my cells recover? But once I learnt the tricks, the system works. Compared to centrifugation my yield recovery is 30-70% higher and it’s rapid. Today, it’s my preferred method to trap and isolate cells.
By continuously optimizing his experiments Chris Dunning now has a 90% recovery. A high recovery rate is vital, as for instance spinal fluid from patients is extremely precious.
All fluids can be used: tears, blood, spinal fluids, saliva… It’s a good way to get results in a non-evasive manner without having to do a biopsy. The results give a good snapshot of what is happening in cells!
According to Chris Dunning, the perspectives of a nearly fully-automated system are interesting. It’s a time-saver and with only small amounts of material, it holds interesting perspectives for future diagnostics of patients at an earlier stage.