The AcouTrap is a tabletop research platform for acoustic trapping. The central part of the system is an acoustic trapping unit where standing waves are used to trap and hold particles, cells or extracellular vesicles against a fluid flow. The acoustic forces can hold polymer microbeads or cells at sizes down to a few micrometers. For smaller objects, e.g. bacteria, extracellular vesicles or nanoparticles, larger polymer seed particles are initially trapped and smaller objects are drawn to these particles by secondary acoustic forces.
The AcouTrap acoustic cell trapping platform
The AcouTrap platform consists of a motorized stage, syringe pumps and valves to interface the acoustic trapping unit with standard 96-well microplates. The system can aspirate and dispense samples and reagents while keeping your trapped sample immobilized in the trapping unit. After processing the sample, the acoustic forces can be switched off and the sample is dispensed into one of the wells. The system is controlled by a computer through a graphical user interface where it is also possible to create user defined scripts automating the sample handling. PhD Chris Dunning describes how he uses the AcouTrap.
The main advantages of using the AcouTrap system compared to centrifugation is ability to
handle low particle/cell concentrations, automated sample processing, shorter sample
processing times and higher recovery rates.
AcouTrap Product Description, AcouTrap 2.1 (pdf), Please find further details about the AcouTrap including Pumps, Sample handling, Motorized stage and Acoustic trapping unit.
On isolation of extracellular vesicles:
- Automated staining (pdf), on using AcouTrap for automated and rapid sample staining and washing
- Removal of sample contaminants (pdf), comparing the purity of EV samples isolated using AcouTrap or centrifugation
- Exosome isolation (pdf), a comparison between AcouTrap and ultracentrifugation for exosome isolation
- EV recovery and sample sources (pdf), on isolation of extracellular vesicles from plasma, urine and conditioned media and the recovery compared to centrifugation
- Vesicle Capture (pdf), includes details on capture and washing of platelet-derived microparticles from human plasma samples
- Proteomic profiling of extracellular vesicles reveals additional diagnostic biomarkers for myocardial infarction compared to plasma alone. Gidlöf et al., Scientific Reports, 2019, 9, 8777
- A urinary extracellular vesicle microRNA biomarker discovery pipeline; from automated extracellular vesicle enrichment by acoustic trapping to microRNA sequencing. Ku et al., PLoS ONE, 2019, 14(5): e0217507
- Acoustic Enrichment of Extracellular Vesicles from Biological Fluids. Ku et al., Anal. Chem. 2018, 90, 13, 8011-8019
- Effect of Exercise on the Plasma Vesicular Proteome: a Methodological Study Comparing Acoustic Trapping and Centrifugation. Bryl-Górecka et al., Lab Chip, 2018, 18, 3101-3111
- Comparative Proteomic Analysis of Extracellular Vesicles Isolated by Acoustic Trapping or Differential Centrifugation. Rezeli et al., Anal. Chem. 2016, 88, 17, 8577-8586
- Non-contact Acoustic Capture of Microparticles from Small Plasma Volumes. Evander et al., Lab Chip, 2015,15, 2588-2596